@article{YANG2024, 
author = {Shushu YANG and Dong LIU and Jie LI and Mengjun ZHANG and Li WANG},
title = {Characterization of CD8+ T cell subsets in male and female non-obese diabetic mice},
year = {2024},
journal = {Journal of Army Medical University},
volume = {46},
number = {8},
pages = {837-848},
keywords = {type 1 diabetes, CD8 positive T lymphocytes, Treg cells, exhausted T cells, naive T cells, memory T cells},
url = {https://www.sciopen.com/article/10.16016/j.2097-0927.202312058},
doi = {10.16016/j.2097-0927.202312058},
abstract = {ObjectiveTo compare and analyze the differences in CD8+ naive, effector, memory, exhausted and regulatory T cells in order to investigate the impact of gender on the differentiation fate of CD8+ T cells in the context of type 1 diabetes (T1D) based on female and male non-obese diabetic (NOD) mice and healthy Institute for Cancer Research (ICR) mice.MethodsThe frequencies and phenotypes of CD8+ T cell differentiation subsets including naive T cells (TN), central memory T cells (TCM), effector T cells (TEFF), effector precursor T cells (TEP), exhausted T cells (TEX), precursor exhausted T cells (TPEX) and regulatory T cells (Tregs) in the spleen, pancreatic draining lymph nodes (pLN) and pancreas infiltrating lymphocytes (PIL) of male and female NOD mice were detected by flow cytometry.ResultsThe frequencies of IFN-γ+, CD107a+ and CCL5+ CD8+ TEFF in pLN and PIL of female NOD mice were significantly higher than those of male NOD mice. However, the frequencies of CD8+ TN, CD8+ TCM, CD8+ TEX, CD8+ TPEX and CD122+CD8+ Tregs subsets in the spleen were significantly decreased. While there were no significant differences in the above CD8+ T cell subsets except CD8+ Tregs between female and male ICR mice.ConclusionAndrogen may inhibit the differentiation of memory T cells into effector T cells and promote the exhaustion of effector T cells, leading to the difference in morbidity between the male and female mice.}
}