@article{LIAO2024, 
author = {Xianjian LIAO and Jing WEN and Jiaxiang DUAN and Lunli XIANG and Zhen YANG and Qingying HE and Jiaolin NING},
title = {Effects and mechanism of methionine restriction on macrophage for lipopolysaccharide-induced acute lung injury in mice},
year = {2024},
journal = {Journal of Army Medical University},
volume = {46},
number = {7},
pages = {688-694},
keywords = {acute lung injury, macrophage, methionine restriction, chemokines, lipopolysaccharide},
url = {https://www.sciopen.com/article/10.16016/j.2097-0927.202308112},
doi = {10.16016/j.2097-0927.202308112},
abstract = {ObjectiveTo investigate the effects of methionine restriction (MR) on macrophages in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and to explore the underlying mechanism.MethodsAccording to the random number table method, 36 male C57BL/6J mice (6~8 weeks old, 23±2 g) were divided into 3 groups with 12 mice in each group: the sham group, the LPS group and the LPS+MR group. HE staining and pathological scoring of lung injury were performed in lung tissues. The expression of LPS-binding protein (LBP) and Toll-like receptor-4 (TLR4) was detected by RT-qPCR and Western blotting. Macrophage-colony stimulating factor (M-CSF), granulocyte-macrophage-colony stimulating factor (GM-CSF) and chemokine C-C motif ligand 3(CCL3) which are all macrophage-associated chemokines were analyzed by immunohistochemistry.ResultsCompared with the sham group, the pathological score of lung injury in the LPS group was significantly increased (P < 0.01); The mRNA and protein expression levels of LBP and TLR4 were significantly increased; The number of positive cells of CD11b, F4/80, M-CSF, GM-CSF and CCL3 were significantly increased (P < 0.01). MR significantly improved LPS-induced ALI, and decreased the pathological score of lung injury (P < 0.01); The mRNA and protein expression levels of LBP and TLR4 were decreased; Compared with the LPS group, the number of positive cells of CD11b, F4/80, M-CSF, GM-CSF and CCL3 were reduced in the LPS+MR group (P < 0.01).ConclusionMR could attenuate LPS-induced ALI by inhibiting the expression of macrophage chemokines and preventing infiltration and activation of macrophage to lungs.}
}