@article{WANG2024, 
author = {Shun WANG and Lin JIANG and Qi HE and Hao YANG and Yiying WANG and Yuning ZHOU and Xin LIANG and Yi ZHANG and Fenglei CHAO and Lei ZHANG and Yong TANG},
title = {Effects of hippocampal overexpression of LINGO-1 on learning and memory abilities and Spinophilin+ dendritic spines in different hippocampal subregions of mice},
year = {2024},
journal = {Journal of Army Medical University},
volume = {46},
number = {2},
pages = {118-127},
keywords = {hippocampus, memory, learning, LINGO-1, dendritic spine, synapses},
url = {https://www.sciopen.com/article/10.16016/j.2097-0927.202306100},
doi = {10.16016/j.2097-0927.202306100},
abstract = {ObjectiveTo investigate the effects of adenovirus-associated virus (AAV)-mediated overexpression of LINGO-1 in the hippocampus on spatial learning and memory abilities, as well as the volume and Spinophilin+ dendritic spines of different subregions within the hippocampus in mice.MethodsMale C57 mice at 7 months of age were randomly divided into a control group and a LINGO-1 overexpression group. The control group received hippocampal stereotaxic injection of AAV carrying green fluorescent protein (GFP), while the LINGO-1 overexpression group received hippocampal stereotaxic injection of AAV carrying both GFP and LINGO-1 overexpression vector. Morris water maze was used to evaluate spatial learning and memory abilities of the mice. Quantitative PCR and immunofluorescence staining were used to detect LINGO-1 mRNA expression and fluorescence intensity in the hippocampus, and 3-dimensional stereology was used to quantify the volume and the total number of Spinophilin+ dendritic spines.ResultsThere was no significant difference in body weight between the control group and the LINGO-1 overexpression group before and after virus injection. LINGO-1 overexpression induced an increase in hippocampal LINGO-1 at mRNA level and in fluorescent intensity(P < 0.01). The LINGO-1 overexpression group performed significantly worse in the Morris water maze test compared to the control group(P < 0.05). Moreover, LINGO-1 overexpression led to a significant reduction in the volumes of different hippocampal subregions(P < 0.05, P < 0.01), accompanied by marked decreases in the density(P < 0.01, P < 0.05) and total number of Spinophilin+ dendritic spines(P < 0.05).ConclusionStereotaxic injection of AAV-mediated LINGO-1 overexpression specifically upregulates LINGO-1 in the hippocampus of mice. Abnormally high expression of LINGO-1 in the hippocampus leads to decreased hippocampal volume and loss of Spinophilin+ dendritic spines, as well as impaires spatial learning and memory abilities of mice to a certain extent.}
}