@article{YANG2024, 
author = {Lanqing YANG and Na WU and Penghui CHEN and Zhiquan YUAN and Chengying LI and Long WU and Li ZHONG and Yafei LI},
title = {Circular RNA mmu_circ_0005019 regulates small conductance calcium-activated potassium current and action potential duration in mouse cardiomyocytes},
year = {2024},
journal = {Journal of Army Medical University},
volume = {46},
number = {2},
pages = {100-109},
keywords = {circular RNA, cardiomyocyte, small conductance calcium-activated potassium current, action potential duration, patch clamp, },
url = {https://www.sciopen.com/article/10.16016/j.2097-0927.202306024},
doi = {10.16016/j.2097-0927.202306024},
abstract = {ObjectiveTo investigate the effect of mmu_circ_0005019 on regulating Kcnn3 expression, small conductance calcium-activated potassium current (IK,Ca) which is generated by the small conductance calcium-activated potassium (SK) channel that is partly coded by Kcnn3, and action potential duration (APD).MethodsWe transfected pcDNA3.1-mmu_circ_0005019 expressing vectors and 2 independent small interfering RNAs into HL-1 cells to establish gain- and loss-of-expression cell models, respectively. The transfected cells were divided into overexpression group(n=3), empty vector group(n=3), interference 1 group(n=3), interference 2 group(n=3), and blank control group(n=3). RT-qPCR and Western blotting were used to determine the expression of Kcnn3. Whole-cell patch clamp technology was performed to record the IK, Ca and APD. The effects of mmu_circ_0005019 on Kcnn3 expression, IK, Ca and APD were observed.ResultsGain- and loss-of-expression cell models were established successfully. The expression of Kcnn3 and IK,Ca current density significantly increased, and the APD was shortened in overexpression group compared with empty vector group(P < 0.05). The expression of Kcnn3 and IK,Ca current density were significantly decreased, and the APD was prolonged in interference groups compared with blank control group(P < 0.05).ConclusionMmu_circ_0005019 promotes the expression of Kcnn3 and its coding channel protein, which regulates IK,Ca and APD in mouse cardiomyocytes, and may play a promoting role in the occurrence of atrial fibrillation.}
}