@article{LIU2024, 
author = {Hongzhen LIU and Yangjie XU and Qin ZHAO and Guanghui QI and Haiyan WANG and Sen LI and Yuan LIU and Botao TAN and Ying YIN and Ce YANG},
title = {Establishment and identification of a rat model of low back pain induced by simulated helicopter low-frequency vibration},
year = {2024},
journal = {Journal of Army Medical University},
volume = {46},
number = {1},
pages = {58-65},
keywords = {low-frequency vibration, acute low back pain, animal model, pain-related behaviors, multifidus muscle},
url = {https://www.sciopen.com/article/10.16016/j.2097-0927.202307074},
doi = {10.16016/j.2097-0927.202307074},
abstract = {ObjectiveTo establish an acute low back pain (ALBP) model by simulating low-frequency vibration of helicopters and explore the causes of ALBP in army aviation pilots in order to provide a reliable animal model and evaluation method for its pathogenesis and protection.MethodsForty-eight male SD rats (8 weeks old, weighing 200±20 g) were selected and randomly divided into groups A, B and C, with vibration for 1, 3 and 6 h, respectively, and group D as blank control, with 12 rats in each group. The rats were fixed in sitting posture on a vibrating table (10 Hz, with 6 degree vibration) for corresponding durations during 5 consecutive days. Animal behavioral tests were performed before and on days 1, 4, 7 and 14 after modelling, including paw withdrawal threshold, open field test, rotarod fatigue test, gait analysis, and 24-hour food intake assessment. Finally, light microscopy was used to observe the morphological structure of the multifidus muscle.ResultsBehavioral examinations revealed that persistent low-frequency vibration resulted in decreased foot-contraction reflex thresholds (P < 0.01), amount of 24-hour food consumption (P < 0.01), count of upright standing (P < 0.05), rotarod velocity at rat falling off (P < 0.05), duty cycle (P < 0.05), footprint surface area (P < 0.05), and walking speed (P < 0.05). Histological observation for the multifidus muscle demonstrated cellular edema and myocyte disorganization accompanied by inflammatory cell infiltration and aggregation.ConclusionContinuous exposure to a low-frequency vibration leads to significant low back pain-related behaviors and histological changes in the lumbar multifidus muscle of rats.}
}