TY  - JOUR
AU  - WANG, Huan
AU  - ZENG, Qiyao
AU  - WANG, Chunsheng
AU  - GUO, Junjie
AU  - ZENG, Jie
PY  - 2023
TI  - High-quality tissue culture via seed embryos of Terminalia bellirica
JO  - Journal of Central South University of Forestry & Technology
SN  - 1673-923X
SP  - 53
EP  - 61,88
VL  - 43
IS  - 9
AB  - ObjectiveThe purpose of this study was to explore the optimal medium components at each stage of tissue culture and the effect of potassium silicate on the height uniformity of plantlets, and a high-quality tissue culture system of Terminalia bellirica was established, so as to provide theoretical and technical support for the breeding and targeted cultivation of T. bellirica.MethodUsing seeds of the elite T. bellirica family as the material, the optimal disinfection method and suitable medium components for proliferation and rooting were screened, and the regulation mechanism of potassium silicate on plantlet uniformity was explored. The suitable seed disinfection methods and proliferation and rooting media components were determined by analyzing differences in seed disinfection effect, proliferation quality and rooting situation among different treatments.ResultThe proper seed disinfection method was soaking the seeds with concentrated sulfuric acid for 7 h and sterilizing the seed embryos with 0.1% mercury solutions for 8 min. In this way, the germination rate of aseptic embryos could exceed 80%. In the proliferation culture, when the ratio of 6-BA to IBA was 10, and the concentrations of KH2PO4 and MgSO4·7H2O were 2 times and 1.5 times that of MS medium, respectively, the proliferation effect of T. bellirica was the best, with a high proliferation coefficient and good growth state. The proliferation coefficient, plantlet height and chlorophyll content could reach up to 5.6, 3.75 cm and 2.51mg·g-1FW, respectively, after 30 days of inoculation. The optimal proliferation medium for T. bellirica was selected as a modified MS medium containing 1.0 mg·L-1 6-BA, 0.1 mg·L-1 IBA, 30 g·L-1 sucrose, 255 mg·L-1 KH2PO4 and 740 mg·L-1 MgSO4·7H2O. In addition, we found that potassium silicate could improve the uniformity of the proliferated plantlets, and its optimal concentration was 1.5 mmol·L-1. The uniformity was 2.63 times higher than that of the control. The best medium for rooting was the Wood Plant Medium supplemented with 0.2 mg·L-1 NAA and 20 g·L-1 sucrose, and the rooting rate could be above 94%.ConclusionThe tissue culture system of T. bellirica was completely established in the present study. In particular, it is the first report on improving the uniformity of T. bellirica plantlets through adding suitable concentrations of potassium silicate into the proliferation medium. The system can not only obtain high-quality plantlets with high height, chlorophyll content and proliferation coefficients, but also improve the uniformity of proliferated plantlets, and reduce the cost for plantlet production, which provides technical support for tissue culture and micro-propagation of the elite T bellirica germplasm.
UR  - https://doi.org/10.14067/j.cnki.1673-923x.2023.09.005
DO  - 10.14067/j.cnki.1673-923x.2023.09.005