TY  - JOUR
AU  - Yang, Jiakang
AU  - Shuai, Jing
AU  - Siow, Lixuen
AU  - Lu, Jingyi
AU  - Sun, Miao
AU  - An, Wenyue
AU  - Yu, Mengfei
AU  - Wang, Baixiang
AU  - Chen, Qianming
PY  - 2024
TI  - MicroRNA-146a-loaded magnesium silicate nanospheres promote bone regeneration in an inflammatory microenvironment
JO  - Bone Research
SN  - 2095-4700
SP  - 2
VL  - 12
AB  - Reconstruction of irregular oral-maxillofacial bone defects with an inflammatory microenvironment remains a challenge, as chronic local inflammation can largely impair bone healing. Here, we used magnesium silicate nanospheres (MSNs) to load microRNA-146a-5p (miR-146a) to fabricate a nanobiomaterial, MSN+miR-146a, which showed synergistic promoting effects on the osteogenic differentiation of human dental pulp stem cells (hDPSCs). In addition, miR-146a exhibited an anti-inflammatory effect on mouse bone marrow-derived macrophages (BMMs) under lipopolysaccharide (LPS) stimulation by inhibiting the NF-κB pathway via targeting tumor necrosis factor receptor-associated factor 6 (TRAF6), and MSNs could simultaneously promote M2 polarization of BMMs. MiR-146a was also found to inhibit osteoclast formation. Finally, the dual osteogenic-promoting and immunoregulatory effects of MSN+miR-146a were further validated in a stimulated infected mouse mandibular bone defect model via delivery by a photocuring hydrogel. Collectively, the MSN+miR-146a complex revealed good potential in treating inflammatory irregular oral-maxillofacial bone defects.
UR  - https://doi.org/10.1038/s41413-023-00299-0
DO  - 10.1038/s41413-023-00299-0