@article{Zhang2024, 
author = {Xu Zhang and Lijuan Zhu and Ming Qian and Li Jiang and Peng Gu and Luting Jia and Chunlu Qian and Weiqi Luo and Min Ma and Zhangfei Wu and Xin Qiao and Libin Wang and Shaoling Zhang},
title = {PbrWRKY62-PbrADC1 module involves in superficial scald development of Pyrus bretschneideri Rehd.fruit via regulating putrescine biosynthesis},
year = {2024},
journal = {Molecular Horticulture},
volume = {4},
number = {1},
pages = {6},
keywords = {P. bretschneideri Rehd., Superficial scald development, Putrescine biosynthesis, PbrWRKY62-PbrADC1 module, Gene expression, Metabolomics},
url = {https://www.sciopen.com/article/10.1186/s43897-024-00081-8},
doi = {10.1186/s43897-024-00081-8},
abstract = {Putrescine plays a role in superficial scald development during the cold storage of pear fruit. However, the molecular mechanism behind this phenomenon has not been un-fully clarified until recently. In this study, a conjoint analysis of metabolites and gene expression profiles in the putrescine-metabolic pathway of P. bretschneideri Rehd. fruit followed by experimental validation revealed that PbrADC1, forming a homodimer in the chloroplast, was involved in putrescine biosynthesis and thus fruit chilling resistance. Additionally, the substrate-binding residue Cys546 in PbrADC1, whose activity was modified by H2O2, played a crucial role in arginine decarboxylation into agmatine. Through a combined analysis of the distribution of cis-acting elements in the PbrADC1 promoter as well as the expression profiles of related transcription factors (TFs), several TFs were identified as upstream regulators of PbrADC1 gene. Further investigation revealed that the nuclear PbrWRKY62 could directly bind to the W-box elements in the PbrADC1 promoter, activate its expression, enhance putrescine accumulation, and thus increase fruit chilling tolerance. In conclusion, our results suggest that the PbrWRKY62-PbrADC1 module is involved in the development of superficial scald in P. bretschneideri Rehd. fruit via regulating putrescine biosynthesis. Consequently, these findings could serve as valuable genetic resources for breeding scald-resistant pear fruit.}
}