@article{Lin2025, 
author = {Shuhua Lin and Shuhong Zheng and Yanquan Chen and Xixi Cai and Shaoyun Wang},
title = {Zinc-binding peptides inactivate Pseudomonas aeruginosa via disrupting membrane and interfering with quorum-sensing},
year = {2025},
journal = {Food Science and Human Wellness},
volume = {14},
number = {9},
pages = {9250315},
keywords = {Crimson snapper scales, CSSP-Zn, Strain PAO1, Membrane disruption mechanism, Quorum sensing},
url = {https://www.sciopen.com/article/10.26599/FSHW.2024.9250315},
doi = {10.26599/FSHW.2024.9250315},
abstract = {Pseudomonas aeruginosa, recognized for its biofilm production and secretion of virulence factors, posing a severe threat in areas such as clinical infections, food contamination, and marine biofouling. To address this, a new type of zinc-binding peptide (CSSP-Zn) was prepared from crimson snapper scales peptides (CSSP) and goslarite, and its antibacterial and anti-quorum-sensing activities toward P. aeruginosa PAO1 were exploited. Results indicated that CSSP-Zn induced planktonic strain PAO1 membrane injury via inhibiting expression levels of cell integrity genes, targeting microbial-specific membrane constituents, disrupting proton motive force, and causing metabolic disturbances. Meanwhile, CSSP-Zn decreased virulence factors pyocyanin, protease and rhamnolipid secretion, while considerably inhibiting quorum sensing-related genes (las, pqs and rhl) expression and decreasing bacterial abundance and pathogenicity in fish models. Moreover, CSSP-Zn not only effectively hindered biofilm formation but also disassembled preformed ones, thus disrupting biofilm topology. Taken together, utilizing food byproducts to obtain CSSP-Zn could help recycle food resources and provide insight into controlling planktonic and biofilm strain PAO1 contamination.}
}