@article{Chen2025, 
author = {Chunlan Chen and Fengqi Zhou and Hongmin Zhu and Ding Ma and Yufan Huang and Jing Li and Linjiang Guo and Lin Xiao and Guobao Ning and Xiaokang Lu and Bilian Li and Hui Zhao and Canpeng Li},
title = {Tri-signal colorimetric/electrochemical detection of egg allergen based on boric acid affinity reagent modified CuO nanoparticles},
year = {2025},
journal = {Food Science and Human Wellness},
volume = {14},
number = {6},
pages = {9250330},
keywords = {Tri-signal detection, Colorimetric/electrochemical, Ovomucoid, CuO nanozyme, Glycoprotein},
url = {https://www.sciopen.com/article/10.26599/FSHW.2024.9250330},
doi = {10.26599/FSHW.2024.9250330},
abstract = {A tri-signal ultrasensitive colorimetric/electrochemical detection of ovomucoid (OM) was developed. Herein, copper oxide nanoparticles (CuO NPs) were prepared, which exhibit excellent enzyme-like activity (peroxidase-like and laccase-like) and electrochemical activity. CuO@3-APBA nanoparticles (CuO@3-APBA NPs) were prepared by the coordinating Cu with the amino group on 3-aminophenobenic boric acid (3-APBA) in CuO NPs. 3-APBA of CuO@3-APBA can react with diol structure on sugar chain of OM under alkaline conditions. Then, a tri-signal ultrasensitive biosensing platform for OM was established based on the catalytic activity of CuO@3-APBA nanozyme. For the first signal, CuO@3-APBA can catalyze oxidation of 1,3,5-trimethylbenzene (TMB) to turn the solution from colorless to blue in the presence of H2O2 (absorbance at 652 nm). For the second signal, CuO@3-APBA can catalyze the oxidation of substrates (2, 4-dichlorophenol and 4-aminoantipyrine) and turn the solution from colorless to pink (absorbance at 510 nm). For the third signal, electrochemical oxidation peak of copper ion from Cu+ to Cu2+ of CuO@3-APBA was recorded by differential pulse voltammetry, which was used to determine the OM. The sensing platform exhibited a wide linear range (0.0000316–100.000000ng/mL) with a low detection limit (0.0105 pg/mL), as well as showed advantages, such as satisfactory reproducibility, good stability, and excellent selectivity. The assay has the potential applications for ultrasensitive detection of allergen in foods.}
}