@article{Nowicka2024, 
author = {Anna Nowicka and Martin Kovacik and Anna Maksylewicz and Przemysław Kopeć and Ewa Dubas and Monika Krzewska and Agnieszka Springer and Robert E. Hoffie and Diaaeldin S. Daghma and Zbyněk Milec and Ales Pecinka and Jochen Kumlehn and Iwona Żur},
title = {The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley},
year = {2024},
journal = {The Crop Journal},
volume = {12},
number = {4},
pages = {1064-1080},
keywords = {Hordeum vulgare, Marker gene, Microspore embryogenesis, Stress response, Transcriptome analysis},
url = {https://www.sciopen.com/article/10.1016/j.cj.2024.07.003},
doi = {10.1016/j.cj.2024.07.003},
abstract = {Plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental factors. Haploids/doubled haploids (DHs) derived from in vitro-cultured microspores are widely used in plant breeding and genetic engineering. However, the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined. Here, RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis (ME) in barley cultivars Golden Promise and Igri, which exhibit contrasting responsiveness to microspore-derived plant formation. Our experiments revealed fundamental regulatory networks, specific groups of genes, and transcription factor (TF) families potentially regulating the developmental switch. We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME. Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.}
}