@article{Wang2024, 
author = {Yao Wang and Dongwei Wang and Kai Wang and Min Zhao and Cizhou Li and Yu Wang and Xuwei Liu and Lei Zhao and Zhuoyan Hu},
title = {γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {5},
pages = {3043-3053},
keywords = {Gut microbiota, Anti-inflammation, Litchi thaumatin-like protein, Mitogen-activated protein kinase pathway, γ-Aminobutyric acid},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250251},
doi = {10.26599/FSHW.2022.9250251},
abstract = {Previous research reported litchi thaumatin-like protein (LcTLP) could lead to inflammation, which is a factor causing the adverse reactions after excessive intake of litchi. As a main amino acid in litchi pulp, γ-aminobutyric acid (GABA) was found with anti-inflammatory effect. Therefore, this study aimed to investigate the effects of GABA on LcTLP-induced inflammation through RAW264.7 macrophages and C57BL mice models. In vitro study showed GABA could effectively regulate the level of inflammatory cytokines (interleukin (IL)-1β, IL-6, IL-10, and prostaglandin E2) and Ca2+ in cells, and inhibit the phosphorylation of p65, I κB, p38, c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). These results indicate GABA alleviated inflammation through nuclear factor-κB and mitogen-activated protein kinase pathway signaling pathways. In vivo experiment was performed to verify the anti -inflammatory effect of GABA, and the results demonstrated that GABA reduced the inflammation and oxidative stress in the liver of LcTLP-treated mice, as it down-regulated the pro- inflammatory cytokines, malondialdehyde, aspartate transferase, and alanine transaminase. The relative expression of phosphorylated p38, JNK and ERK in mice liver with GABA treatment were reduced to 65%, 39% and 80% of the control group, respectively. Furthermore, GABA treatment enriched probiotic bacteria and decreased pathogenic bacteria in mice gut, which reveals GABA could effectively reduce the translocation of gut microbiota.}
}