@article{Yan2024, 
author = {Hongzhang Yan and Hong Shen and Jinrong SiTu and Yingying Yang and Lingle Zhang and Kai Yang},
title = {Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer},
year = {2024},
journal = {Nano Biomedicine and Engineering},
volume = {16},
number = {3},
pages = {416-428},
keywords = {malignant tumor, cationic liposome, As@LNPs, siRNA, Bmi-1, drug delivery},
url = {https://www.sciopen.com/article/10.26599/NBE.2024.9290086},
doi = {10.26599/NBE.2024.9290086},
abstract = {Malignant tumors remain a serious threat to human health and life and are a major public health problem globally. Herein, we designed and synthesized a novel nucleic acid nanomedicine AS1411-siRNA-LNPs (As@LNPs). Bmi-1 siRNA was coated with cationic liposomes, and a nucleic acid aptamer AS1411 with tumor cell-targeting ability was attached to the outermost layer of the liposomes. The average particle size of As@LNPs was 183 nm, and the polydispersion coefficient was 0.187. The encapsulation rate and drug loading of As@LNPs were 85% and 4.6%, respectively. The average electron mobility of the drug was 2.64 (μ/s)/(V/cm), and the zeta potential of As@LNPs was 33.79 ± 0.78 mV. The microstructure of the nanomedicine was evaluated via transmission electron microscopy. In vitro and in vivo experiments showed that As@LNPs significantly inhibited tumor growth and promoted tumor cell apoptosis. As@LNPs showed favorable biosafety with major tissues and organs, except glomerulus and renal epithelial cells.}
}