@article{Tu2024, 
author = {Ranran Tu and Hong Wang and Zhihao Sun and Qinwen Zou and Jiajun Wu and Duo Wu and Nan Wang and Guanghua He},
title = {A rice exocyst subunit gene SEC3A is required for anther dehiscence},
year = {2024},
journal = {The Crop Journal},
volume = {12},
number = {3},
pages = {938-943},
keywords = {Rice, Male sterility, Anther dehiscence, Exocyst, SEC3A},
url = {https://www.sciopen.com/article/10.1016/j.cj.2024.04.010},
doi = {10.1016/j.cj.2024.04.010},
abstract = {Anther dehiscence controls optimal interaction between pollen and stigma, thereby determining the successful sexual reproduction. The regulators or mechanisms of this process remain elusive. Here, two CRISPR/Cas9 mutants of a rice exocyst subunit gene SEC3A, sec3a-1 and sec3a-2, showed anther indehiscence at anthesis and male sterility at maturity. Pollen viability and germination in the mutants were partly defective, whereas their female gametes undergone a normal development. Hybrid or self-pollinated seeds could be produced by artificial pollination, suggesting potential use of a weak sec3a mutant as a female line during hybrid breeding. SEC3A is widely expressed in various tissues, including anther walls. Further results showed an excessive IAA accumulation and no endothecium lignification in sec3a-1/2 anthers. Our findings suggest that SEC3A appears to regulate anther dehiscence by modulating auxin signaling, providing insights into regulation of anther dehiscence and function of exocyst in plants.}
}