@article{Wu2025, 
author = {Yanyan Wu and Huifang Wang and Huan Xiang and Di Wang and Chunsheng Li and Shengjun Chen and Yongqiang Zhao and Yueqi Wang},
title = {Lipidomics and whole-genome sequencing identify lipid profiles in fermented golden pompano (Trachinotus ovatus) inoculated with Bacillus subtilis: influence of indigenous strains on lipid changes},
year = {2025},
journal = {Food Science and Human Wellness},
volume = {14},
number = {6},
pages = {9250141},
keywords = {Untargeted lipidomics, Golden pompano, Fermentation, Lipase, Whole genome},
url = {https://www.sciopen.com/article/10.26599/FSHW.2024.9250141},
doi = {10.26599/FSHW.2024.9250141},
abstract = {Inoculated fermentation enables rapid fermentation of aquatic products. No studies have been conducted on the lipid profiles of inoculated fermented golden pompano (Trachinotus ovatus). In this study, a lipase-producing Bacillus subtilis with salt tolerance was screened from traditionally fermented golden pompano (TF) and used as a starter culture. Whole-genome sequencing analysis revealed it carries 4 clustered regularly interspaced short vpalindromic repeats structures and 2 genes encoding triacylglycerol lipase. Untargeted lipidomics identified lipid molecules (833) in 6 major classes from B. subtilis SCSMX-2 fermented golden pompano (IF). A total of 28 lipid molecules were upregulated in IF, including phosphatidylcholines (PCs), triacylglycerols (TAGs), and lysophosphatidylcholine. B. subtilis supplementation enhanced the production of polyenyl PCs and medium- and long-chain TAGs. The IF rich in linoleic, docosahexaenoic acids (DHA), and eicosapentaenoic acid were primarily distributed in the sn-2 position of DHA, PC and phosphatidylethanolamine. This research revealed the lipid profiles of IF, providing theoretical basis for the application of B. subtilis in the fermented fish industry.}
}