@article{Sun2024, 
author = {Yudie Sun and Yunxiang Han and Mingyue Wang and Mingfu Ye and Konglin Wu and Kui Zhang},
title = {An ultra-sensitive biosensor for circulating microRNA detection with Fe single-atom enhanced cathodic luminol-O2 electrochemiluminescence},
year = {2024},
journal = {Nano Research},
volume = {17},
number = {9},
pages = {7926-7934},
keywords = {single-atom catalyst, electrochemiluminescence, hybridization chain reaction, circulating miRNAs, luminol-O2},
url = {https://www.sciopen.com/article/10.1007/s12274-024-6767-2},
doi = {10.1007/s12274-024-6767-2},
abstract = {Circulating microRNAs (miRNAs) play a pivotal role in the occurrence and development of acute myocardial infarction (AMI), and precise detection of them holds significant clinical implications. The development of luminol-based luminophores in the field of electrochemiluminescence (ECL) for miRNA detection has been significant, while their effectiveness is hindered by the instability of co-reactant hydrogen peroxide (H2O2). In this work, an iron single-atom catalyst (Fe-PNC) was employed for catalyzing the luminol-O2 ECL system to achieve ultra-sensitive detection of myocardial miRNA. Target miRNA triggers a hybridization chain reaction (HCR), resulting in the generation of a DNA product featuring multiple sticky ends that facilitate the attachment of Fe-PNC probes to the electrode surface. The Fe-PNC catalyst exhibits high promise and efficiency for the oxygen reduction reaction (ORR) in electrochemical energy conversion systems. The resulting ECL biosensor allowed ultrasensitive detection of myocardial miRNA with a low detection limit of 0.42 fM and a wide linear range from 1 fM to 1.0 nM. Additionally, it demonstrates exceptional performance when evaluated using serum samples collected from patients with AMI. This work expands the application of single-atom catalysis in ECL sensing and introduces novel perspectives for utilizing ECL in disease diagnosis.}
}