@article{Ahirwar2016, 
author = {Saroj Ahirwar and Hemant Soni and Hemant Kumar Rawat and Bhanu Pratap Prajapati and Naveen Kango},
title = {Experimental design of response surface methodology used for utilisation of palm kernel cake as solid substrate for optimised production of fungal mannanase},
year = {2016},
journal = {Mycology},
volume = {7},
number = {3},
pages = {143-153},
keywords = {Agro-waste, fungi, palm kernel cake, β-mannanase, response surface methodology},
url = {https://www.sciopen.com/article/10.1080/21501203.2016.1229697},
doi = {10.1080/21501203.2016.1229697},
abstract = {The results obtained from this work strongly indicate that the solid state fermentation (SSF) system using the palm kernel cake (PKC) as a substrate is an economical method for the production of β-mannanase at extremely low operational cost based on the fact that PKC is one of the cheap and abundant agro-waste by-products of the palm oil industry. Under initial conditions, i.e. 2 mm particle size of PKC, the moisture ratio of 1:1 of PKC:moistening agent and pH 7, Malbranchea cinnamomea NFCCI 3724 produced 109 U/gram distribution of the substrate (gds). The production of β-mannanase was optimised by the statistical approach response surface methodology (RSM) using independent variables, namely initial moisture (12.5), pH (9.0) and solka floc (100 mg). Noticeably, six fold enhancement of β-mannanase production (599 U/gds) was obtained under statistically optimised conditions. HPLC results revealed that β-mannanase is an endo-active enzyme that generated manno-oligosaccharides with a degree of polymerisation (DP) of 3 and 4. Semi-native PAGE analysis revealed that M. cinnamomea produced three isoforms of mannanase. Selective production of oligosaccharide makes M. cinnamomea β-mannanase an attractive enzyme for use in food and nutraceutical industries.}
}