TY  - JOUR
AU  - Ridha, Dalal Mohammed
AU  - AL-Rafyai, Hawraa Mohammed
AU  - Saleh, Mustafa Abed AL-Jabber Mohammed
PY  - 2025
TI  - Evaluation of the Antibacterial Impact of the Conjugated Gentamycin-biosynthesized Silver Nanoparticles on Resistant Pseudomonas aeruginosa Isolates In Vitro
JO  - Nano Biomedicine and Engineering
SN  - 2097-3837
SP  - 551
EP  - 560
VL  - 17
IS  - 4
AB  - The expanding resistance of pathogenic bacteria to antibiotics casts a serious threat to the public health. Thus, a new strategy is required to resolve this problem. This study aims to compare the antibacterial impact of biosynthesized silver nanoparticles (Bio-AgNPs), gentamycin (GEN), and the conjugation of biosynthesized silver nanoparticles and gentamycin (GEN:Bio-AgNPs) on multidrug-resistant Pseudomonas aeruginosa isolates. The characteristic properties of Bio-AgNPs were detected by the following analyses: ultraviolet–visible (UV–Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Depending on micro-dilution assay, the minimum inhibitory concentration (MIC) of the tested subjects (Bio-AgNPs, GEN, GEN:Bio-AgNPs) are calculated to be 112 ± 400, 1 536 ± 525, and 49 ± 18.8 µg/mL, respectively. The obtained results confirmed that the GEN:Bio-AgNPs have greater potency effect than Bio-AgNPs and GEN alone, showing that AgNPs of low concentration can potentially enhance the effect of GEN against resistant P. aeruginosa.
UR  - https://doi.org/10.26599/NBE.2024.9290067
DO  - 10.26599/NBE.2024.9290067