@article{Shen2024, 
author = {Liping Shen and Lili Zhang and Changbin Yin and Xiaowan Xu and Yangyang Liu and Kuocheng Shen and He Wu and Zhiwen Sun and Ke Wang and Zhonghu He and Xueyong Zhang and Chenyang Hao and Jian Hou and Aoyue Bi and Xuebo Zhao and Daxing Xu and Botao Ye and Xuchang Yu and Ziying Wang and Danni Liu and Yuanfeng Hao and Fei Lu and Zifeng Guo},
title = {The wheat sucrose synthase gene TaSus1 is a determinant of grain number per spike},
year = {2024},
journal = {The Crop Journal},
volume = {12},
number = {1},
pages = {295-300},
keywords = {Domestication selection, Fertile spikelet number per spike (FSN), Geographical differentiation, Grain number per spike (GNS), TaSus1},
url = {https://www.sciopen.com/article/10.1016/j.cj.2023.11.007},
doi = {10.1016/j.cj.2023.11.007},
abstract = {Some haplotypes of the sucrose synthase gene TaSus1 are associated with thousand-grain weight (TGW) in wheat (Triticum aestivum L.). However, no mutations have been identified within the gene to test this association. The effects of TaSus1 on grain number per spike (GNS) also are largely unknown. Our previous genome-wide association study identified TaSus-A1 as a candidate gene controlling fertile spikelet number per spike (FSN). In the present study, we generated two independent mutants for the three TaSus1 homoeologs by CRISPR/Cas9-mediated genome editing. The triple mutants displayed lower FSN, GNS, grain number per spikelet (GNST), and TGW than wild-type plants. In 306 hexaploid wheat accessions, two single-nucleotide polymorphisms in TaSus-A1 contributed differently to GNS. Introgression of the two alleles into a wheat genetic background confirmed their effects. The alleles differed in geographical distribution among the accessions.}
}