@article{Hong2024, 
author = {Zishan Hong and Jing Xie and Liang Tao and Jing-Jing Dai and Tingting Li and Li Zhang and Yuying Bai and Xia Hu and Jinlian Chen and Jun Sheng and Yang Tian},
title = {Exploration of cyclooxygenase-2 inhibitory peptides from walnut dreg proteins based on in silico and in vitro analysis},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {3},
pages = {1636-1644},
keywords = {Walnut dreg proteins, Cyclooxygenase-2 inhibitory peptide, Identification, Virtual screening, Molecular docking},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250143},
doi = {10.26599/FSHW.2022.9250143},
abstract = {Walnut dreg protein hydrolysates (WDPHs) exhibit a variety of biological activities, however, the cyclooxygenase-2 (COX-2) inhibitory peptide of WDPHs remain unclear. The aim of this study was to rapidly screen for such peptides in WDPHs through a combination of in silico and in vitro analysis. In total, 1262 peptide sequences were observed by nano liquid chromatography/tandem mass spectrometry (nano LC-MS/MS) and 4 novel COX-2 inhibitory peptides (AGFP, FPGA, LFPD, and VGFP) were identif ied. Enzyme kinetic data indicated that AGFP, FPGA, and LFPD displayed mixed-type COX-2 inhibition, whereas VGFP was a non-competitive inhibitor. This is mainly because the peptides form hydrogen bonds and hydrophobic interactions with residues in the COX-2 active site. These results demonstrate that computer analysis combined with in vitro evaluation allows for rapid screening of COX-2 inhibitory peptides in walnut protein dregs.}
}