TY  - JOUR
AU  - Yang, Shiqiang
AU  - Chen, Yexin
AU  - Huan, Fei
AU  - He, Xinrong
AU  - Yun, Xiao
AU  - Liu, Hong
AU  - Chen, Guixia
AU  - Liu, Guangming
PY  - 2024
TI  - Identification and analysis of immunological activity of two isoforms of tropomyosin in Alectryonella plicatula
JO  - Food Science and Human Wellness
SN  - 2097-0765
SP  - 3697
EP  - 3707
VL  - 13
IS  - 6
AB  - Oyster, as a common aquatic food, play an important role in shellfish allergy. In this study, 2 tropomyosin (TM) isoforms TM-α and TM-β (TM-α/-β) in Alectryonella plicatula were identified. The sequences of 852 bp encoding 284 amino acids of TM-α/-β and 2 recombinant proteins were obtained, respectively. There were 12 amino acid differences between TM-α/-β. The results of immunological experiments indicated that TM-β had stronger immunobinding activity and immunoreactivity than those of TM-α. Structural analysis showed that TM-β had more α-helix and higher surface hydrophobicity than TM-α. Sequences and epitopes alignment with shellfish TMs revealed that amino acids of TM-β were more frequently recognized as IgE epitopes in other shellfish TMs than TM-α. Differences in structure and sequence account for the higher immunological activity of TM-β compared to TM-α. These findings provide a theoretical basis for enriching the understanding of shellfish TM and accurate diagnosis of allergic components.
UR  - https://doi.org/10.26599/FSHW.2023.9250050
DO  - 10.26599/FSHW.2023.9250050