@article{Karki2018, 
author = {Namrata Karki and Sita Aggarwal and Frank Greenway and Roger A. Laine and Jack N. Losso},
title = {Juglone down-regulates the Akt-HIF-1α and VEGF signaling pathways and inhibits angiogenesis in MIA Paca-2 pancreatic cancer in vitro},
year = {2018},
journal = {Journal of Food Bioactives},
volume = {1},
pages = {143-152},
keywords = {Juglone, angiogenesis, pancreatic cancer, HIF-1α, VEGF, p-AkT},
url = {https://www.sciopen.com/article/10.31665/JFB.2018.1133},
doi = {10.31665/JFB.2018.1133},
abstract = {Hypoxia-inducible factor -1 alpha (HIF-1α), vascular endothelial growth factor (VEGF), and phosphorylated Akt (p-Akt) are critical in pancreatic cancer cell growth, angiogenesis and metastasis. The ability of MIA Paca-2 pancreatic cancer cells to migrate and invade after treatment with juglone (5 hydroxy-1, 4-naphthoquinone) was analyzed by a transwell invasion and migration assay, a wound healing assay, and an in vitro tube formation assay using human umbilical vein endothelial cells (HUVEC). ELISA was used to determine the levels of HIF1-α. Western blot analysis was used to determine the level of VEGF, p-Akt, and carbonic anhydrase IX expression. Juglone down-regulated the expression of HIF-1α, VEGF, and significantly suppressed the phosphorylation of Akt. Juglone dose-dependently inhibited the metastatic potential of pancreatic cancer cells by inhibiting cell migration, invasion and wound healing assays. Juglone attenuated the aggressiveness of pancreatic cancer cells in vitro.}
}