@article{Zhou2023, 
author = {Ming Zhou and Yaxue Li and Xing Lu and Panmin He and Cuiyue Liang and Jiang Tian},
title = {Diverse functions of GmNLA1 members in controlling phosphorus homeostasis highlight coordinate response of soybean to nitrogen and phosphorus availability},
year = {2023},
journal = {The Crop Journal},
volume = {11},
number = {4},
pages = {1251-1260},
keywords = {GmNLA1, N availability, P deficiency, P homeostasis, Soybean},
url = {https://www.sciopen.com/article/10.1016/j.cj.2022.12.003},
doi = {10.1016/j.cj.2022.12.003},
abstract = {Nitrogen (N) and phosphorus (P) are two essential mineral nutrients for plant growth, which are required in relative high amount in plants. Plants have evolved a series of strategies for coordinately acquiring and utilizing N and P. However, physiological and molecular mechanisms underlying of N and P interactions remain largely unclear in soybean (Glycine max). In this study, interactions of N and P were demonstrated in soybean as reflected by significant increases of phosphate (Pi) concentration in both leaves and roots by N deficiency under Pi sufficient conditions. A total of four nitrogen limitation adaptation (NLA), encoding RING-type E3 ubiquitin ligase were subsequently identified in soybean genome. Among them, transcription of GmNLA1-1 and GmNLA1-3 was decreased in soybean by N starvation under Pi sufficient conditions, not for GmNLA1-2. Suppression of all three GmNLA1 members was able to increase Pi concentration regardless of the P and N availability in the growth medium, but decrease fresh weight under normal conditions in soybean hairy roots. However, comparted to changes in control lines at two N levels, N deficiency only resulted in a relatively higher increase of Pi concentration in GmNLA1-1 or GmNLA1-3 suppression lines, strongly indicating that GmNLA1-1 and GmNLA1-3 might regulate P homeostasis in soybean response to N starvation. Taken together, our result suggest that redundant and diverse functions present in GmNLA1 members for soybean coordinate responses to P and N availability, which mediate P homeostasis.}
}