@article{Chen2024, 
author = {Yuzhou Chen and Mo Chen and Chengxuan Yu and Huizhu Li and Liman Sai and Nguyen T. K. Thanh and Yueming Wang and Yan Wo and Jian Zhang and Xing Yang and Evgenii L. Guryev and Andrei V. Zvyagin and Hao De and Min Tang and Shiyi Chen and Yunxia Li and Yuefeng Hao and Sijia Feng and Jun Chen},
title = {In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging},
year = {2024},
journal = {Nano Research},
volume = {17},
number = {5},
pages = {4379-4390},
keywords = {cell therapy, in vivo imaging, macrophage, NIR-II, delayed therapy, tendon healing},
url = {https://www.sciopen.com/article/10.1007/s12274-023-6363-x},
doi = {10.1007/s12274-023-6363-x},
abstract = {The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage therapy. In this work, the second near-infrared (NIR-II) fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their administration. The delayed cell therapy (DCT) group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy (ICT) group. Both Keller–Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with ICT. Our results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.}
}