@article{Dai2014, 
author = {Yuntao Dai and Eugenia Winston and James C. Correll and Yulin Jia},
title = {Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae},
year = {2014},
journal = {The Crop Journal},
volume = {2},
number = {1},
pages = {1-9},
keywords = {Avirulence gene, AVR-Pita1, Pi-ta, Rice blast resistance},
url = {https://www.sciopen.com/article/10.1016/j.cj.2013.12.002},
doi = {10.1016/j.cj.2013.12.002},
abstract = {The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that originally were collected from the U.S. and are virulent on Pi-ta-carrying rice cultivars. The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe. The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta. Control isolates that were transformed with inserts lacking the AVR-Pita1 gene remained virulent. Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature.}
}