@article{Zhang2017, 
author = {Congsheng Zhang and Changlin Liu and Jianfeng Weng and Beijiu Cheng and Fang Liu and Xinhai Li and Chuanxiao Xie},
title = {Creation of targeted inversion mutations in plants using an RNA-guided endonuclease},
year = {2017},
journal = {The Crop Journal},
volume = {5},
number = {1},
pages = {83-88},
keywords = {RNA-guided endonuclease, CRISPR/Cas9, Targeted inversion, Genetic improvement},
url = {https://www.sciopen.com/article/10.1016/j.cj.2016.08.001},
doi = {10.1016/j.cj.2016.08.001},
abstract = {Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.}
}