@article{Pan2024, 
author = {Chuang Pan and Xiaofan Zhang and Shengjun Chen and Yong Xue and Yanyan Wu and Yueqi Wang and Di Wang},
title = {Analysis of quality-related proteins in golden pompano (Trachinotus ovatus) fillets with modifi ed atmosphere packaging under superchilling storage},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {4},
pages = {2253-2265},
keywords = {Low-field nuclear magnetic resonance, Modified atmosphere packaging, Superchilling storage, Protein deterioration, Tandem mass tags (TMT) proteomics, Trachinotus ovatus},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250188},
doi = {10.26599/FSHW.2022.9250188},
abstract = {Here, we aimed to study the changes in proteome of golden pompano fillets during post-mortem storage. Tandem mass tags (TMT) -labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging (MAP) fillets during superchilling (-3 °C) storage. Scanning electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees, and low-field nuclear magnetic resonance was used to indf that the immobilized water and free water in the muscle of fillets changed significantly. Total sulfhydryl content, TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and denaturation. The Fresh (FS), MAP, and air packaging (AP) groups were set. Total of 150 proteins were identified as differential abundant proteins (DAPs) in MAP/FS, while 209 DAPs were in AP/FS group. The KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein turnover. Correlation analysis found that 52 DAPs were correlated with quality traits. Among them, 8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding capacity. These results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.}
}