@article{Hu2024, 
author = {Tenggen Hu and Yuanshan Yu and Jijun Wu and Yujuan Xu and Gengsheng Xiao and Kejing An and Erna Li and Sentai Liao and Yuxiao Zou},
title = {Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {4},
pages = {2161-2173},
keywords = {Mulberry leaf oligosaccharide, Characterization, Gut microbiota, Lactobacillus murinus, Hypoglycemic activity},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250180},
doi = {10.26599/FSHW.2022.9250180},
abstract = {Two oligosaccharide fractions (MLO 2-1 and 2-2) were purified from enzymatic hydrolysate of mulberry leaf polysaccharide. The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide, which could be degraded by human digestive juice; while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota. Hence, MLO 2-1 was selected for the further analysis. The structure of MLO 2-1 was elucidated as follow: α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1. The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota. Meanwhile, MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids, especially acetate and butyrate. The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated. Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus, a commensal bacterium presented a reduced level in T2DM mice. Animal experiments indicated that MLO 2-1 and L. murinus exhibited hypoglycemic activities. These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L. murinus.}
}