@article{Chen2018, 
author = {Li Chen and Yupeng Cai and Xiujie Liu and Chen Guo and Shi Sun and Cunxiang Wu and Bingjun Jiang and Tianfu Han and Wensheng Hou},
title = {Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation},
year = {2018},
journal = {The Crop Journal},
volume = {6},
number = {2},
pages = {162-171},
keywords = {Agrobacterium rhizogenes, Hairy root, Protocol, Soybean, Transformation},
url = {https://www.sciopen.com/article/10.1016/j.cj.2017.08.006},
doi = {10.1016/j.cj.2017.08.006},
abstract = {Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10–12days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.}
}