TY  - JOUR
AU  - Chen, Li
AU  - Cai, Yupeng
AU  - Liu, Xiujie
AU  - Guo, Chen
AU  - Sun, Shi
AU  - Wu, Cunxiang
AU  - Jiang, Bingjun
AU  - Han, Tianfu
AU  - Hou, Wensheng
PY  - 2018
TI  - Soybean hairy roots produced in vitro by Agrobacterium rhizogenes-mediated transformation
JO  - The Crop Journal
SN  - 2095-5421
SP  - 162
EP  - 171
VL  - 6
IS  - 2
AB  - Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10–12days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.
UR  - https://doi.org/10.1016/j.cj.2017.08.006
DO  - 10.1016/j.cj.2017.08.006