@article{Xu2019, 
author = {Dongdong Xu and Dan Sun and Yanling Diao and Minxuan Liu and Jia Gao and Bin Wu and Xingmiao Yuan and Ping Lu and Zongwen Zhang and Jing Zhang and Ganggang Guo},
title = {Fast mapping of a chlorophyll b synthesis-deficiency gene in barley (Hordeum vulgare L.) via bulked-segregant analysis with reduced-representation sequencing},
year = {2019},
journal = {The Crop Journal},
volume = {7},
number = {1},
pages = {58-64},
keywords = {Barley, BSA-seq, Reduced representative sequencing, Pale-green, Chlorophyllide a oxygenase},
url = {https://www.sciopen.com/article/10.1016/j.cj.2018.07.002},
doi = {10.1016/j.cj.2018.07.002},
abstract = {Bulked-segregant analysis coupled with next-generation sequencing (BSA-seq) has emerged as an efficient tool for genetic mapping of single genes or major quantitative trait loci controlling (agronomic) traits of interest. However, such a mapping-by-sequencing approach usually relies on deep sequencing and advanced statistical methods. Application of BSA-Seq based on construction of reduced-representation libraries and allele frequency analysis permitted anchoring the barley pale-green (pg) gene on chromosome 3HL. With further marker-assisted validation, pg was mapped to a 3.9 Mb physical-map interval. In the pg mutant a complete deletion of chlorophyllide a oxygenase (HvCAO) gene was identified. Because the product of this gene converts Chl a to Chl b, the pg mutant is deficient in Chl b. An independent Chl b-less mutant line M4437_2 carried a nonsynonymous substitution (F263L) in the C domain of HvCAO. The study demonstrates an optimized pooling strategy for fast mapping of agronomically important genes using a segregating population.}
}