@article{Wang2022, 
author = {Chu-Kun Wang and Xiu-Ming Li and Fang Dong and Cui-Hui Sun and Wen-Li Lu and Da-Gang Hu},
title = {Yang cycle enzyme DEP1: its moonlighting functions in PSI and ROS production during leaf senescence},
year = {2022},
journal = {Molecular Horticulture},
volume = {2},
number = {2},
pages = {10},
keywords = {Photosynthesis, ROS, Leaf senescence, DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1, Yang cycle, Photosystem Ⅰ},
url = {https://www.sciopen.com/article/10.1186/s43897-022-00031-2},
doi = {10.1186/s43897-022-00031-2},
abstract = {Ethylene-mediated leaf senescence and the compromise of photosynthesis are closely associated but the underlying molecular mechanism is a mystery. Here we reported that apple DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1 (MdDEP1), initially characterized to its enzymatic function in the recycling of the ethylene precursor SAM, plays a role in the regulation of photosystem Ⅰ (PSI) activity, activating reactive oxygen species (ROS) homeostasis, and negatively regulating the leaf senescence. A series of Y2H, Pull-down, CO-IP and Cell-free degradation biochemical assays showed that MdDEP1 directly interacts with and dephosphorylates the nucleus-encoded thylakoid protein MdY3IP1, leading to the destabilization of MdY3IP1, reduction of the PSI activity, and the overproduction of ROS in plant cells. These findings elucidate a novel mechanism that the two pathways intersect at MdDEP1 due to its moonlighting role in destabilizing MdY3IP1, and synchronize ethylene-mediated leaf senescence and the compromise of photosynthesis.}
}