TY  - JOUR
AU  - Wang, Lingshuang
AU  - Lin, Chun
AU  - Li, Bohui
AU  - Su, Tong
AU  - Li, Shichen
AU  - Li, Haiyang
AU  - He, Fanglei
AU  - Gou, Chuanjie
AU  - Chen, Zheng
AU  - Wang, Yanan
AU  - Qin, Jun
AU  - Liu, Baohui
AU  - Kong, Fanjiang
AU  - Yue, Lin
AU  - Lu, Sijia
AU  - Fang, Chao
PY  - 2023
TI  - Two soybean homologues of TERMINAL FLOWER 1 control flowering time under long day conditions
JO  - The Crop Journal
SN  - 2095-5421
SP  - 704
EP  - 712
VL  - 11
IS  - 3
AB  - Flowering time is a key agronomic trait that directly affect the adaptation and yield of soybean. After whole genome duplications, about 75% of genes being represented by multiple copies in soybean. There are four TERMINAL FLOWER 1 (TFL1) genes in soybean, and the TFL1b (Dt1) has been characterized as the determinant of stem growth habit. The function of other TFL1 homologs in soybean is still unclear. Here, we generated knockout mutants by CRISPR/Cas9 genome editing technology and found that the tfl1c/tfl1d double mutants flowered significantly earlier than wild-type plants. We investigated that TFL1c and TFL1d could physically interact with the bZIP transcription factor FDc1 and bind to the promoter of APETALA1a (AP1a). RNA-seq and qRT-PCR analyses indicated that TFL1c and TFL1d repressed the expressions of the four AP1 homologs and delayed the flowering time in soybean. The two genes play important roles in the regulation of flowering time in soybean and mainly act as the flowering inhibitors under long-day conditions. Our results identify novel components in the flowering-time regulation network of soybean and will be invaluable for molecular breeding of improved soybean yield.
UR  - https://doi.org/10.1016/j.cj.2023.01.008
DO  - 10.1016/j.cj.2023.01.008