@article{Chen2021, 
author = {Xiao Chen and Suxin Yang and Yaohua Zhang and Xiaobin Zhu and Xinjing Yang and Chunbao Zhang and Haiyan Li and Xianzhong Feng},
title = {Generation of male-sterile soybean lines with the CRISPR/Cas9 system},
year = {2021},
journal = {The Crop Journal},
volume = {9},
number = {6},
pages = {1270-1277},
keywords = {CRISPR/Cas9, Soybean, Tapetum, Gmams, Male sterility},
url = {https://www.sciopen.com/article/10.1016/j.cj.2021.05.003},
doi = {10.1016/j.cj.2021.05.003},
abstract = {Soybean [Glycine max (L.) Merr.] provides a rich source of plant protein and oil worldwide. The commercial use of transgenic technology in soybean has become a classical example of the application of biotechnology to crop improvement. Although genetically modified soybeans have achieved commercial success, hybrid soybean breeding is also a potential way to increase soybean yield. Soybean cytoplasmic male-sterile (CMS) lines have been used in three-line hybrid breeding systems, but their application to exploiting soybean heterosis has been limited by rare germplasm resource of sterile lines. The generation of various genetic diversity male-sterile soybean lines will help to overcome the shortcoming. In this study, we used targeted editing of AMS homologs in soybean by CRISPR/Cas9 technology for the first time to generate stable male-sterile lines. Targeted editing of GmAMS1 resulted in a male-sterile phenotype, while editing of GmAMS2 failed to produce male-sterile lines. GmAMS1 functions not only in the formation of the pollen wall but also in the controlling the degradation of the soybean tapetum. CRISPR/Cas9 technology could be used to rapidly produce stable male-sterile lines, providing new sterile-line materials for soybean hybrid breeding systems.}
}