TY  - JOUR
AU  - Zhang, Bo
AU  - Qi, Feixiang
AU  - Hu, Gang
AU  - Yang, Yikai
AU  - Zhang, Li
AU  - Meng, Jianghu
AU  - Han, Zhongmin
AU  - Zhou, Xiangchun
AU  - Liu, Haiyang
AU  - Ayaad, Mohammed
AU  - Xing, Yongzhong
PY  - 2021
TI  - BSA-seq-based identification of a major additive plant height QTL with an effect equivalent to that of Semi-dwarf 1 in a large rice F2 population
JO  - The Crop Journal
SN  - 2095-5421
SP  - 1428
EP  - 1437
VL  - 9
IS  - 6
AB  - Bulked-segregant analysis is a time- and cost-saving strategy for identifying major quantitative trait loci (QTL) in a mapping population. Bulked-segregant analysis combined with whole-genome sequencing (BSA-seq) was performed to rapidly identify QTL for heading date, plant height, and panicle length in a large F2 population derived from two landraces: Chuan 7 (C7) and Haoboka (HBK). Twenty plants with extremely low or high phenotypic values for the target traits were selected from an F2 population of 940 plants to construct low- and high-value bulks. Three pairs of bulks for the three traits were constructed, resulting in six DNA pools. BSA-seq revealed nine QTL: four for heading date, three for plant height, and two for panicle length. These QTL were validated in a random F2 population or BC4F2 populations. The major novel plant height QTL, qPH8, acting additively with an effect equivalent to that of semi-dwarf 1 (sd1), is potentially valuable for hybrid rice breeding. qPH8 controls mainly the elongation of basal internodes. The C7 allele of qPH8 reduces plant height and increases lodging resistance without yield penalty, suggesting a potential role for qPH8 in improving rice plant architecture.
UR  - https://doi.org/10.1016/j.cj.2020.11.011
DO  - 10.1016/j.cj.2020.11.011