@article{Qin2020, 
author = {Ruiying Qin and Shengxiang Liao and Juan Li and Hao Li and Xiaoshuang Liu and Jianbo Yang and Pengcheng Wei},
title = {Increasing fidelity and efficiency by modifying cytidine base-editing systems in rice},
year = {2020},
journal = {The Crop Journal},
volume = {8},
number = {3},
pages = {396-402},
keywords = {CRISPR-Cas9, Base editing, BE3, CDA, Oryza sativa},
url = {https://www.sciopen.com/article/10.1016/j.cj.2019.04.007},
doi = {10.1016/j.cj.2019.04.007},
abstract = {The efficiency of plant cytidine base-editing systems is limited, and unwanted mutations frequently occur in transgenic plants. We increased the cytidine editing frequency and fidelity of the plant base editor 3 (BE3) and targeted activation-induced cytidine deaminase (CDA) (target-AID) systems by coexpressing three copies of free uracil–DNA glycosylase (UDG) inhibitor (UGI). The editing efficiency of the improved BE3 and CDA systems reached as high as 88.9% and 85.7%, respectively, in regenerated rice plants, with a very low frequency of unwanted mutations. The low editing frequency of the BE3 system in the GC context could be overcome by the modified CDA system. These results provide a high-fidelity and high-efficiency solution for rice genomic base editing.}
}