@article{Wang2017, 
author = {Shengliu Wang and Shuoguo Li and Gang Ji and Xiaojun Huang and Fei Sun},
title = {Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ},
year = {2017},
journal = {Biophysics Reports},
volume = {3},
number = {1-3},
pages = {8-16},
keywords = {Correlative cryo-light and electron microscopy, iCorr, Mitochondria, Primary hippocampal neuron cell, Syntaphilin},
url = {https://www.sciopen.com/article/10.1007/s41048-017-0035-x},
doi = {10.1007/s41048-017-0035-x},
abstract = {Correlative cryo-fluorescence and cryo-electron microscopy (cryo-CLEM) system has been fast becoming a powerful technique with the advantage to allow the fluorescent labeling and direct visualization of the close-to-physiologic ultrastructure in cells at the same time, offering unique insights into the ultrastructure with specific cellular function. There have been various engineered ways to achieve cryo-CLEM including the commercial FEI iCorr system that integrates fluorescence microscope into the column of transmission electron microscope. In this study, we applied the approach of the cryo-CLEM-based iCorr to image the syntaphilin-immobilized neuronal mitochondria in situ to test the performance of the FEI iCorr system and determine its correlation accuracy. Our study revealed the various morphologies of syntaphilin-immobilized neuronal mitochondria that interact with microtubules and suggested that the cryo-CLEM procedure by the FEI iCorr system is suitable with a half micron-meter correlation accuracy to study the cellular organelles that have a discrete distribution and large size, e.g. mitochondrion, Golgi complex, lysosome, etc.}
}