@article{Jin2019, 
author = {Lei Jin and Li Kou and Yanan Zeng and Chunguang Hu and Xiaodong Hu},
title = {Sample preparation method to improve the efficiency of high-throughput single-molecule force spectroscopy},
year = {2019},
journal = {Biophysics Reports},
volume = {5},
number = {4},
pages = {176-183},
keywords = {Sample preparation, Specific binding, Digital holographic centrifugal force microscopy, High-throughput single-molecule force spectroscopy},
url = {https://www.sciopen.com/article/10.1007/s41048-019-00097-4},
doi = {10.1007/s41048-019-00097-4},
abstract = {Inefficient sample preparation methods hinder the performance of high-throughput single-molecule force spectroscopy (H-SMFS) for viscous damping among reactants and unstable linkage. Here, we demonstrated a sample preparation method for H-SMFS systems to achieve a higher ratio of effective target molecules per sample cell by gas-phase silanization and reactant hydrophobization. Digital holographic centrifugal force microscopy (DH-CFM) was used to verify its performance. The experimental result indicated that the DNA stretching success ratio was improved from 0.89% to 13.5%. This enhanced efficiency preparation method has potential application for force-based DNA stretching experiments and other modifying procedures.}
}