TY  - JOUR
AU  - Jin, Lei
AU  - Kou, Li
AU  - Zeng, Yanan
AU  - Hu, Chunguang
AU  - Hu, Xiaodong
PY  - 2019
TI  - Sample preparation method to improve the efficiency of high-throughput single-molecule force spectroscopy
JO  - Biophysics Reports
SN  - 2364-3439
SP  - 176
EP  - 183
VL  - 5
IS  - 4
AB  - Inefficient sample preparation methods hinder the performance of high-throughput single-molecule force spectroscopy (H-SMFS) for viscous damping among reactants and unstable linkage. Here, we demonstrated a sample preparation method for H-SMFS systems to achieve a higher ratio of effective target molecules per sample cell by gas-phase silanization and reactant hydrophobization. Digital holographic centrifugal force microscopy (DH-CFM) was used to verify its performance. The experimental result indicated that the DNA stretching success ratio was improved from 0.89% to 13.5%. This enhanced efficiency preparation method has potential application for force-based DNA stretching experiments and other modifying procedures.
UR  - https://doi.org/10.1007/s41048-019-00097-4
DO  - 10.1007/s41048-019-00097-4