@article{Li2022, 
author = {Mengjiao Li and Tian Wang and Hui Zhang and Shuo Liu and Wenhu Li and Salah F. Abou Elwafa and Hui Tian},
title = {TaNRT2.1-6B is a dual-affinity nitrate transporter contributing to nitrogen uptake in bread wheat under both nitrogen deficiency and sufficiency},
year = {2022},
journal = {The Crop Journal},
volume = {10},
number = {4},
pages = {993-1005},
keywords = {Candidate gene association study, Wheat, TaNRT2.1-6B, Dual-affinity transporter, Nitrogen use efficiency},
url = {https://www.sciopen.com/article/10.1016/j.cj.2021.11.012},
doi = {10.1016/j.cj.2021.11.012},
abstract = {Multiple nitrate transporter (NRT) genes exist in the genome of bread wheat, and it is of great importance to identify the elite NRT genes for N-efficient wheat cultivar breeding. A candidate gene association study (CGAS) of six N use efficiency (NUE) related traits (grain N concentration (GNC), straw N concentration (SNC), grain yield (GY), grain N accumulation (GNA), shoot total N accumulation (STN) and N harvest index (NHI)) was performed based on SNPs in 46 NRT2 genes using a panel composed of 286 wheat cultivars. CGAS identified TaNRT2.1-6B as an elite NRT gene that is significantly associated with four (NHI, SNC, GNA and GY) of the six NUE-related traits simultaneously. TaNRT2.1-6B is located on the plasma membrane and acts as a dual-affinity NRT. The overexpression of TaNRT2.1-6B increased the N influx and root growth of wheat, whereas gene silence lines resulted in the opposite effects. The overexpression of TaNRT2.1-6B also improved GY and N accumulation of wheat under either limited or sufficient N conditions. The data provide the TaNRT2.1-6B gene and the two associated SNP markers as promising powerful tools for breeding wheat cultivars with high N uptake ability and NUE.}
}