@article{Feng2022, 
author = {Xuanjun Feng and Yue Hu and Weixiao Zhang and Rongqian Xie and Huarui Guan and Hao Xiong and Li Jia and Xuemei Zhang and Hanmei Zhou and Dan Zheng and Ying Wen and Qingjun Wang and Fengkai Wu and Jie Xu and Yanli Lu},
title = {Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding},
year = {2022},
journal = {The Crop Journal},
volume = {10},
number = {4},
pages = {1213-1218},
keywords = {P5CS, Proline, Drought, Tiller number, Chalkiness},
url = {https://www.sciopen.com/article/10.1016/j.cj.2022.04.002},
doi = {10.1016/j.cj.2022.04.002},
abstract = {The delta-1-pyrroline-5-carboxylate synthetase (P5CS) gene exercises a protective function in stressed plants. However, the relationship between proline accumulation caused by P5CS and abiotic stress tolerance in plants is not always clear, as P5CS overexpression has been reported to repress plant growth under normal conditions in several reports. We re-evaluated the role of P5CS in drought-tolerant rice breeding by expressing the AtP5CS1 and feedback-inhibition-removed AtP5CS1 (AtP5CS1F128A) genes under the regulation of an ABA-inducible promoter to avoid the potential side effects of P5CS overexpression under normal conditions. ABA-inducible AtP5CS1 and AtP5CS1F128A increased seedling growth in a nutrient solution (under osmotic stress) and grain yield in pot plants. However, the evidently deleterious effects of AtP5CS1 on grain quality, tiller number, and grain yield in the field indicated the unsuitability of P5CS for drought-tolerance breeding.}
}