@article{Dai2024, 
author = {Zhuqing Dai and Meimei Nie and Ye Chen and Jiangfeng Song and Yayuan Xu and Zhongyuan Zhang and Guodong Zhang and Shumo Yan and Xing Zhang and Dajing Li},
title = {Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {3},
pages = {1628-1635},
keywords = {Lutein, Stevioside, Antioxidant, Human retinal pigment epithelial cell, Mechanism},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250138},
doi = {10.26599/FSHW.2022.9250138},
abstract = {In order to improve the bioavailability of lutein (LUT), a novel lutein-stevioside nanoparticle (LUT-STE) were prepared previously, but the information about LUT-STE on protecting of eye health was limited. This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial (ARPE) cells. LUT and LUT-STE (final concentration of 5 μg/mL) significantly enhanced cell viability from (74.84 ± 5.10)% to (81.92 ± 10.01)% (LUT) and (89.33 ± 4.34)% (LUT-STE), and inhibited the cell apoptosis (P < 0.05). After pretreatment with LUT-STE in ARPE cells, the levels of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in ARPE cells were significantly increased (P < 0.05), the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were decreased. In addition, the vascular endothelial growth factor (VEGF) levels were inhibited by 13.61% and 17.39%, respectively, pretreatment with LUT and LUT-STE. Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis. In summary, the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells.}
}