@article{Yuan2024, 
author = {Fuhuan Yuan and Yu Fu and Liang Ma and Hankun Zhu and Yong Yu and Xin Feng and Yi Sun and Hongjie Dai and Xin Liu and Zhengfang Liu and Yuhao Zhang},
title = {Calcium-chelating peptides from rabbit bone collagen:characterization, identif ication and mechanism elucidation},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {3},
pages = {1485-1493},
keywords = {Rabbit bone, Collagen peptide, Peptide-calcium chelate, Chelation mechanism, Liquid chromatography-tandem mass (LC-MS/MS)},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250125},
doi = {10.26599/FSHW.2022.9250125},
abstract = {This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism. Collagen peptides and calcium were extracted from rabbit bone by instant ejection steam explosion (ICSE) combined with enzymatic hydrolysis, followed by chelation reaction to prepare rabbit bone peptide-calcium chelate (RBCP-Ca). The chelating sites were further analyzed by liquid chromatography-tandem mass (LC-MS/MS) spectrometry while the chelating mechanism and binding modes were investigated. The structural characterization revealed that RBCP successfully chelated with calcium ions. Furthermore, LC-MS/MS analysis indicated that the binding sites included both acidic amino acids (Asp and Glu) and basic amino acids (Lys and Arg). Interestingly, three binding modes, namely Inter-Linking, Loop-Linking and Mono-Linking were for the first time found, while Inter-Linking mode accounted for the highest proportion (75.1%), suggesting that chelation of calcium ions frequently occurred between two peptides. Overall, this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.}
}