@article{Wang2024, 
author = {Ziyan Wang and Qiuyue Shi and Ying Feng and Jiaojiao Han and Chenyang Lu and Jun Zhou and Zhonghua Wang and Xiurong Su},
title = {Targeted screening of an anti-inflammatory polypeptide from Rhopilema esculentum Kishinouye cnidoblasts and elucidation of its mechanism in alleviating ulcerative colitis based on an analysis of the gut microbiota and metabolites},
year = {2024},
journal = {Food Science and Human Wellness},
volume = {13},
number = {3},
pages = {1336-1347},
keywords = {Ulcerative colitis, Metagenome, Rhopilema esculentum Kishinouye, Cnidoblasts, Marine bioactive polypeptides, Serum metabolome},
url = {https://www.sciopen.com/article/10.26599/FSHW.2022.9250112},
doi = {10.26599/FSHW.2022.9250112},
abstract = {Ulcerative colitis (UC) is a recurrent inflammatory bowel disease that imposes a severe burden on families and society. In recent years, exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research interest. This study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV (Pro-Lys-Lys-Val-Val) of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum metabolome. Specif ically, the polypeptide composition of R. esculentum cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α (TNF-α) than the original ligand. Subsequent animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC; affect serum metabolites through the arachidonic acid, glycerophospholipid and linoleic acid metabolism pathways; and further alleviate UC symptoms. This study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.}
}