@article{Deng2018, 
author = {Huifang Deng and Hong Yang and Dingnan Wang and Baihui Zeng and Shaohua Shi and Han Yu and Yunzhou Li and Zongying Xu and Xianggen Zhong},
title = {Effects of an immunosuppressive active component of Periplocae Cortex (Periploca sepium Bge.) on positive selection of thymocytes in vitro},
year = {2018},
journal = {Journal of Traditional Chinese Medical Sciences},
volume = {5},
number = {2},
pages = {161-167},
keywords = {Immunosuppressive active component, Positive selection, Thymocytes},
url = {https://www.sciopen.com/article/10.1016/j.jtcms.2018.03.002},
doi = {10.1016/j.jtcms.2018.03.002},
abstract = {ObjectiveTo determine the effect of an immunosuppressive active component (periploside A) isolated from the stem bark of Periplocae Cortex (Periploca sepium Bge.), a Chinese medicinal herb used in the treatment of rheumatoid arthritis for centuries in China, on positive selection of thymocytes in vitro.MethodsFemale C57BL/6 mice at 6 weeks of age were housed in specific pathogen-free conditions. Double-positive thymocytes from C57BL/6 mice were induced into positive selection in vitro with or without periploside A treatment. Cell viability and expression of CD69, CD4, and CD8 were analyzed by flow cytometry.ResultsFlow cytometric examination of thymocyte populations revealed that the percentage of CD8+ single-positive thymocytes was decreased by periploside A upon differentiation induced by an anti-CD3 antibody. However, the percentage of CD4+ single-positive thymocytes was decreased by periploside A upon differentiation induced by phorbol 12-myristate 13-acetate/ionomycin. Expression of CD69 plays a major role in prohibiting differentiation of thymocytes. Treatment with periploside A decreased CD69 expression in thymocytes.ConclusionThese results demonstrate that periploside A influences positive selection of thymocytes in vitro.}
}