@article{Wang2023, 
author = {Na Wang and Jun Chen and Yuan Gao and Yongbin Zhou and Ming Chen and Zhaoshi Xu and Zhengwu Fang and Youzhi Ma},
title = {Genomic analysis of isopentenyltransferase genes and functional characterization of TaIPT8 indicates positive effects of cytokinins on drought tolerance in wheat},
year = {2023},
journal = {The Crop Journal},
volume = {11},
number = {1},
pages = {46-56},
keywords = {TaIPT, Phytohormone, Abiotic stress, Cytokinin biosynthesis, Wheat},
url = {https://www.sciopen.com/article/10.1016/j.cj.2022.04.010},
doi = {10.1016/j.cj.2022.04.010},
abstract = {Cytokinins (CKs) function in plant development and during stress responses, but their role in drought tolerance in wheat is unknown. In the present study, 24 isopentenyltransferase (IPT) genes, encoding rate-limiting enzymes in CK biosynthesis were identified in the wheat genome. The chromosomal locations and structures of the genes, protein properties, and phylogenetic relationships were characterized. ATP/ADP TaIPT genes showed tissue-specific expression. TaIPT2, TaIPT7, and TaIPT8 expression was rapidly induced by 0.5–1 h drought treatments, which decreased to low levels after 2 h drought treatment, as did most other TaIPT genes. TaIPT8-5a/5b/5d triple mutants showed decreased levels of tZ-type CK under normal and drought conditions and reduced drought tolerance, which, however, did not manifest as phenotype alterations. By contrast, transgenic wheat plants with drought-induced TaIPT8 showed increased drought tolerance. Our study provides a foundation for further investigation of TaIPT genes and novel insights into the role of CKs in the drought response of wheat.}
}