@article{Liang2023, 
author = {Guihong Liang and Yingpeng Hua and Haifei Chen and Jinsong Luo and Hongkun Xiang and Haixing Song and Zhenhua Zhang},
title = {Increased nitrogen use efficiency via amino acid remobilization from source to sink organs in Brassica napus},
year = {2023},
journal = {The Crop Journal},
volume = {11},
number = {1},
pages = {119-131},
keywords = {Nitrogen, Amino acids, Source-to-sink allocation, Yield, Genetic variation},
url = {https://www.sciopen.com/article/10.1016/j.cj.2022.05.011},
doi = {10.1016/j.cj.2022.05.011},
abstract = {Nitrogen (N) is an essential plant growth nutrient whose coordinated distribution from source to sink organs is crucial for seed development and overall crop yield. We compared high and low N use efficiency (NUE) Brassica napus (rapeseed) genotypes. Metabonomics and transcriptomics revealed that leaf senescence induced by N deficiency promoted amino acid allocation from older to younger leaves in the high-NUE genotype at the vegetative growth stage. Efficient source to sink remobilization of amino acids elevated the numbers of branches and pods per plant under a N-deficiency treatment during the reproductive stage. A 15N tracer experiment confirmed that more amino acids were partitioned into seeds from the silique wall during the pod stage in the high-NUE genotype, owing mainly to variation in genes involved in organic N transport and metabolism. We suggest that the greater amino acid source-to-sink allocation efficiency during various growth stages in the high-NUE genotype resulted in higher yield and NUE under N deficiency. These findings support the hypothesis that strong amino acid remobilization in rapeseed leads to high yield, NUE, and harvest index.}
}