TY  - JOUR
AU  - Liang, Guihong
AU  - Hua, Yingpeng
AU  - Chen, Haifei
AU  - Luo, Jinsong
AU  - Xiang, Hongkun
AU  - Song, Haixing
AU  - Zhang, Zhenhua
PY  - 2023
TI  - Increased nitrogen use efficiency via amino acid remobilization from source to sink organs in Brassica napus
JO  - The Crop Journal
SN  - 2095-5421
SP  - 119
EP  - 131
VL  - 11
IS  - 1
AB  - Nitrogen (N) is an essential plant growth nutrient whose coordinated distribution from source to sink organs is crucial for seed development and overall crop yield. We compared high and low N use efficiency (NUE) Brassica napus (rapeseed) genotypes. Metabonomics and transcriptomics revealed that leaf senescence induced by N deficiency promoted amino acid allocation from older to younger leaves in the high-NUE genotype at the vegetative growth stage. Efficient source to sink remobilization of amino acids elevated the numbers of branches and pods per plant under a N-deficiency treatment during the reproductive stage. A 15N tracer experiment confirmed that more amino acids were partitioned into seeds from the silique wall during the pod stage in the high-NUE genotype, owing mainly to variation in genes involved in organic N transport and metabolism. We suggest that the greater amino acid source-to-sink allocation efficiency during various growth stages in the high-NUE genotype resulted in higher yield and NUE under N deficiency. These findings support the hypothesis that strong amino acid remobilization in rapeseed leads to high yield, NUE, and harvest index.
UR  - https://doi.org/10.1016/j.cj.2022.05.011
DO  - 10.1016/j.cj.2022.05.011