@article{Lei2024, 
author = {Hangbin Lei and Qian Zhang and Xiaoyan Xiang and Liang Jiang and Shiyan Wang and Lingxuan Duan and Shizhen Wang},
title = {Metal-organic framework hybrid materials of ZIF-8/RGO for immobilization of D-amino acid dehydrogenase},
year = {2024},
journal = {Nano Research},
volume = {17},
number = {1},
pages = {290-296},
keywords = {metal-organic frameworks, reduced graphene oxide, immobilization, D-Amino acid dehydrogenase, zeolitic imidazolate framework-8 (ZIF-8)},
url = {https://www.sciopen.com/article/10.1007/s12274-023-5811-y},
doi = {10.1007/s12274-023-5811-y},
abstract = {Immobilization of D-amino acid dehydrogenase (DAADH) by the assembly of peptide linker was studied for the biosynthesis of D-phenylalanine. Hybrid material of zeolitic imidazolate framework-8 (ZIF-8) combined with reduced graphene oxide (RGO) was applied for the immobilization of DAADH from Ureibacillus thermosphaericus. The recovery rate of DAADH/ZIF-8/RGO was 165.6%. DAADH/ZIF-8/RGO remained 53.4% of its initial activity at 50 °C for 10 h while the free enzyme was inactivated. DAADH/ZIF-8/RGO maintained 70.5% activity in hyperalkaline solution with pH 12. Kinetic parameters indicated that DAADH/ZIF-8/RGO had greater affinity of phenylpyruvate as Vmax/Km of DAADH/ZIF-8/RGO was 1.27-fold than free enzyme. After seven recycles, the activity of DAADH/ZIF-8/RGO remained 64.3%. Furthermore, one-step separation and in situ immobilization of DAADH by ZIF-8/RGO/Ni was carried out with 1.5-fold activity enhancement. Combining peptide linker and metal-organic framework (MOF) immobilization, thermostability and activity of the immobilized DAADH were significantly improved.}
}