@article{Pan2022, 
author = {Mingfei Pan and Liping Hong and Jingying Yang and Xiaoqian Xie and Kaixin Liu and Shuo Wang},
title = {Fabrication and evaluation of a portable and reproducible quartz crystal microbalance immunochip for label-free detection of β-lactoglobulin allergen in milk products},
year = {2022},
journal = {Food Science and Human Wellness},
volume = {11},
number = {5},
pages = {1315-1321},
keywords = {β-Lactoglobulin allergen, Quartz crystal microbalance, Immunochip, Milk products},
url = {https://www.sciopen.com/article/10.1016/j.fshw.2022.04.015},
doi = {10.1016/j.fshw.2022.04.015},
abstract = {In this study, a label-free, portable and reproducible immunochip based on quartz crystal microbalance (QCM) was developed for the qualitative detection of β-lactoglobulin (β-LG), an allergen, in milk products. Experimental parameters in the fabrication and regeneration procedure such as pH of the coupling microenvironment, amount of anti-β-LG antibody and regeneration reagent were optimized in detail. Under optimal conditions, the proposed QCM immunochip exhibited good recognition of β-LG, with a calibration curve of ΔF = 12.877Cβ-LG0.4809 (R2 = 0.9982) and limit of detection of 0.04 μg/mL. Additionally, this portable QCM immunochip had good stability, high specificity, and no obvious cross-reaction to three other milk proteins (α-casein, α-lactalbumin, and lactoferrin). It could compete a qualitative measurement within 5 min, and could be reused at least ten times. In the β-LG analysis of actual milk samples, the developed QCM immunochip yielded reliable and accurate results, which correlated strongly with those from the standard HPLC method (R2 = 0.9969). Thus, the portable, stable, and reproducible QCM immunochip developed in this study allowed the rapid, cost-effectively and sensitively measure the β-LG in milk products.}
}